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Biohydrogen Gas/Acetone-Butanol-Ethanol Production from Agave Guishe Juice as a Low-Cost Growing Medium
ALEJANDRA GUADALUPE OLIVA RODRIGUEZ
VIANEY DE JESUS CERVANTES GUICHO
THELMA KARINA MORALES MARTINEZ
JOSE ANTONIO RODRIGUEZ DE LA GARZA
MIGUEL ANGEL MEDINA MORALES
SILVIA YUDITH MARTINEZ AMADOR
ANA GISELA REYES ALVARADO
LEOPOLDO JAVIER RIOS GONZALEZ
Acceso Abierto
Atribución-NoComercial-SinDerivadas
DOI: https://doi.org/10.3390/fermentation9090811
URL: https://www.mdpi.com/2311-5637/9/9/811
ISSN: 2311-5637
Agave lechuguilla, guishe juice, revalorization, Clostridium acetobutylicum, butanol, hydrogen production
"Different strategies have been assessed for the revalorization of guishe to obtain biomolecules. The juice obtained after the mechanical extraction of guishe is rich in phytochemicals and sugars which can be converted to other products. The objective of the present study was to evaluate the production of hydrogen and butanol at different guishe juice concentrations (and therefore, different sugar concentrations) via fermentation in batch mode using Clostridium acetobutylicum ATCC 824. Fermentation assays were performed in triplicate under anaerobic conditions at 35 °C for 142 h. Guishe juice was supplemented with all components of synthetic medium (salts, vitamins and reducing agents), except glucose, and diluted at different concentrations: 20%, 40%, 60%, 80% and 100%. For comparison purposes, a control was carried out in a synthetic medium using glucose as carbon source. Results showed a maximum butanol concentration of 5.39 g/L using 80% guishe juice, corresponding to a productivity and yield of 0.04 g/L h−1 and 0.24 g/g, respectively. Meanwhile, the highest productivity (1.16 L H2/L d−1; 1.99 mmol H2/L h−1) and yield (18.4 L/kg) of hydrogen were obtained with 40% guishe juice. This study demonstrates the potential of guishe juice to be used as a low-cost substrate for hydrogen and butanol production."
Multidisciplinary Digital Publishing Institute
2023
Artículo
Fermentation
Inglés
Oliva-Rodríguez, A.G.; Cervantes-Güicho, V.d.J.; Morales-Martínez, T.K.; Rodríguez-De la Garza, J.A.; Medina-Morales, M.A.; Martínez-Amador, S.Y.; Reyes, A.G.; Ríos-González, L.J. Biohydrogen Gas/Acetone-Butanol-Ethanol Production from Agave Guishe Juice as a Low-Cost Growing Medium. Fermentation 2023, 9, 811. https://doi.org/10.3390/fermentation9090811
BIOQUÍMICA DE ALIMENTOS
Versión publicada
publishedVersion - Versión publicada
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