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The Opuntia streptacantha OpsHSP18 Gene Confers Salt and Osmotic Stress Tolerance in Arabidopsis thaliana
SILVIA SALAS MUÑOZ
GRACIA ALICIA GOMEZ ANDURO
Pablo Delgado Sánchez
Margarita Rodríguez-Kessler
JUAN FRANCISCO JIMENEZ BREMONT
Acceso Abierto
Atribución-NoComercial-SinDerivadas
DOI: doi.org/10.3390/ijms130810154
URL: https://www.mdpi.com/1422-0067/13/8/10154
ISSN: 16616596
abiotic stress, abscisic acid, Arabidopsis thaliana, germination, Opuntia streptacantha, small heat shock protein
"Abiotic stress limits seed germination, plant growth, flowering and fruit quality, causing economic decrease. Small Heat Shock Proteins (sHSPs) are chaperons with roles in stress tolerance. Herein, we report the functional characterization of a cytosolic class CI sHSP (OpsHSP18) from Opuntia streptacantha during seed germination in Arabidopsis thaliana transgenic lines subjected to different stress and hormone treatments. The over-expression of the OpsHSP18 gene in A. thaliana increased the seed germination rate under salt (NaCl) and osmotic (glucose and mannitol) stress, and in ABA treatments, compared with WT. On the other hand, the over-expression of the OpsHSP18 gene enhanced tolerance to salt (150 mM NaCl) and osmotic (274 mM mannitol) stress in Arabidopsis seedlings treated during 14 and 21 days, respectively. These plants showed increased survival rates (52.00 and 73.33%, respectively) with respect to the WT (18.75 and 53.75%, respectively). Thus, our results show that OpsHSP18 gene might have an important role in abiotic stress tolerance, in particular in seed germination and survival rate of Arabidopsis plants under unfavorable conditions."
Multidisciplinary Digital Publishing Institute
2012
Artículo
International Journal of Molecular Sciences
Inglés
Salas-Muñoz, S.; Gómez-Anduro, G.; Delgado-Sánchez, P.; Rodríguez-Kessler, M.; Jiménez-Bremont, J.F. The Opuntia streptacantha OpsHSP18 Gene Confers Salt and Osmotic Stress Tolerance in Arabidopsis thaliana. Int. J. Mol. Sci. 2012, 13, 10154-10175. https://doi.org/10.3390/ijms130810154
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Versión publicada
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