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http://cibnor.repositorioinstitucional.mx/jspui/handle/1001/2579| Development of a LAMP method for detection of carbapenem-resistant Acinetobacter baumannii during a hospital outbreak | |
| CAROLINA GARCIGLIA MERCADO Ramón Gaxiola Robles Felipe de Jesús Ascencio Valle JESUS SILVA SANCHEZ MARIA TERESA ESTRADA GARCIA GRACIA ALICIA GOMEZ ANDURO | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| DOI: 10.3855/jidc.11692 URL: https://jidc.org/index.php/journal/article/view/32525836/2255 ISSN: 19722680 | |
| Acinetobacter baumannii, loop mediated isothermal amplification, carbapenem resistance | |
| "Introduction: Carbapenem-resistant A. baumannii (CRAB) represents a public health threat increasing worldwide. We assess the suitability of a loop-mediated isothermal amplification (LAMP) method for on-site screening of CRAB in a hospital facility. Methodology: A set of six primers were designed for recognizing eight distinct sequences on six targets: blaOXA-23-like, blaOXA-24-like, blaOXA-51- like, blaOXA-58-like, blaIMP, and blaVIM. A LAMP method was developed, optimized and evaluated for the identification of CRAB in thirty-three environmental samples from an outbreak in an Intensive Care Unit (ICU) facility. Results: The sensitivity of the LAMP assay for the detection of A. baumannii was ten-fold higher than the PCR assay (1.0 ng.µL-1). The LAMP assays showed a higher detection rate for CRAB samples and robust diagnosis performance in comparison to a conventional PCR, with clinical sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of 100% for blaOXA-23-like, blaOXA-51-like and blaVIM. Conclusions: The developed LAMP assays are powerful tools that can be useful in on-site screening of CRAB causing local outbreaks in clinics and hospitals facilities where costs and equipment restraints are imperative." | |
| Journal of Infection in Developing Countries | |
| 2020 | |
| Artículo | |
| Journal of Infection in Developing Countries | |
| Inglés | |
| OTRAS | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Artículos |
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| Journal of Infection in Developing Countries 2020 VOL.14NO.5P.494-501.pdf | 82.68 kB | Adobe PDF | Visualizar/Abrir |