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http://cibnor.repositorioinstitucional.mx/jspui/handle/1001/2543| Early Optimization Stages of Agave lechuguilla Bagasse Processing toward Biorefinement: Drying Procedure and Enzymatic Hydrolysis for Flavonoid Extraction | |
| ZOE PELLETIER--MORREEUW LEOPOLDO JAVIER RIOS GONZALEZ CARMEN LIZZETH SALINAS SALAZAR ELDA MADAI MELCHOR MARTINEZ JUAN ALBERTO ASCACIO VALDES ROBERTO PARRA SALDIVAR HAFIZ MUHAMMAD NASIR IQBAL ANA GISELA REYES ALVARADO | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| DOI: 10.3390/ molecules26237292 ISSN: 1420-3049 URL: https://www.mdpi.com/1420-3049/26/23/7292 | |
| agro-industrial waste, biorefinery, added-value natural product, flavonoids, drying process, enzyme-assisted extraction, taguchi method | |
| "Agave lechuguilla agro-waste is a promising renewable material for biorefining purposes. The procurement of added-value co-products, such as bioactive phytochemicals, is required to improve bioprocesses and promote the bio-based economy of the productive areas of Mexico. In this study, we aimed to evaluate the effect of post-harvest management and enzymatic pretreatment as the first stages of the A. lechuguilla valorization process. Four drying methods were compared, and enzymatic hydrolysis was optimized to obtain a flavonoid-enriched extract applying ultrasoundassisted extraction. In both experiments, the total phenolic (TPC) and flavonoid (TFC) contents, HPLC-UV flavonoid profiles, and radical scavenging capacity (DPPH) were considered as response variables. The results demonstrated that light exposure during the drying process particularly affected the flavonoid content, whereas oven-dehydration at 40 ◦C in the dark preserved the flavonoid diversity and antioxidant functionality of the extracts. Flavonoid glycoside recovery, particularly anthocyanidins, was 1.5–1.4-fold enhanced by enzymatic hydrolysis using the commercial mix Ultraflo© under optimized conditions (pH 4, 40 ◦C, 180 rpm, and 2.5 h) compared to the unpretreated biomass. The extraction of flavonoids from A. lechuguilla bagasse can be carried out using a scalable drying method and enzymatic pretreatment. This study confirmed the potential of this agro-waste as a source of marketable natural products." | |
| Multidisciplinary Digital Publishing Institute | |
| 2021 | |
| Artículo | |
| Molecules | |
| Inglés | |
| Morreeuw, Z.P.; Ríos-González, L.J.; Salinas-Salazar, C.; Melchor-Martínez, E.M.; Ascacio-Valdés, J.A.; Parra-Saldívar, R.; Iqbal, H.M.N.; Reyes, A.G. Early Optimization Stages of Agave lechuguilla Bagasse Processing toward Biorefinement: Drying Procedure and Enzymatic Hydrolysis for Flavonoid Extraction. Molecules 2021, 26, 7292. https://doi.org/10.3390/ molecules26237292 | |
| ENZIMOLOGIA | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Artículos |
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