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Effect of Different Species of Prorocentrum Genus on the Japanese Oyster Crassostrea gigas Proteomic Profile
MIGUEL ANGEL MATUS HERNANDEZ
NORMA YOLANDA HERNANDEZ SAAVEDRA
Acceso Abierto
Atribución-NoComercial-SinDerivadas
URL: https://www.mdpi.com/2072-6651/13/7/504
DOI: 10.3390/toxins13070504
ISSN: 2072-6651
Prorocentrum, Crassostrea gigas, proteomic maps, toxin effects, in vitro exposure
"This paper assesses the effects of exposure to toxic concentrations (1200 to 6000 cells/mL) of the dinoflagellates Prorocentrum lima, Prorocentrum minimum, and Prorocentrum rhathymum and several concentrations of aqueous and organic extracts obtained from the same species (0 to 20 parts per thousand) on the Crassostrea gigas (5–7 mm) proteomic profile. Through comparative proteomic map analyses, several protein spots were detected with different expression levels, of which eight were selected to be identified by liquid chromatography–mass spectrometry (LC–MS/MS) analyses. The proteomic response suggests that, after 72 h of exposure to whole cells, the biological functions of C. gigas affected proteins in the immune system, stress response, contractile systems and cytoskeletal activities. The exposure to organic and aqueous extracts mainly showed effects on protein expressions in muscle contraction and cytoskeleton morphology. These results enrich the knowledge on early bivalve developmental stages. Therefore, they may be considered a solid base for new bioassays and/or generation of specific analytical tools that allow for some of the main effects of algal proliferation phenomena on bivalve mollusk development to be monitored, characterized and elucidated."
Multidisciplinary Digital Publishing Institute
2021
Artículo
Toxins
Inglés
Matus Hernández, M.A.; Hernández Saavedra, N.Y. Effect of Different Species of Prorocentrum Genus on the Japanese Oyster Crassostrea gigas Proteomic Profile. Toxins 2021, 13, 504. https:// doi.org/10.3390/toxins13070504
INMUNOLOGÍA
Versión publicada
publishedVersion - Versión publicada
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