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Evaluation of Glycosyl-Hydrolases, Phosphatases, Esterases and Proteases as Potential Biomarker for NaCl-Stress Tolerance in Solanum lycopersicum L. Varieties | |
JUAN JOSE REYES PEREZ FRANCISCO HIGINIO RUIZ ESPINOZA Luis Guillermo Hernández Montiel Barbara De Lucia giuseppe cristiano Bernardo Murillo Amador | |
Acceso Abierto | |
Atribución-NoComercial-SinDerivadas | |
ISSN: 1420-3049 DOI: 10.3390/molecules24132488 URL: https://www.mdpi.com/1420-3049/24/13/2488 | |
salinity-stress tolerance, biochemical indicators, proline, peroxidase, semi-quantitative enzymes, tomato | |
"Salinity stress limited the production in over 30% of irrigated crops and 7% of dryland agriculture worldwide. The objective was to evaluate the e_ects of NaCl-stress on the enzymatic activity in tomato. Twoexperiments were carried out in germination and early vegetative growth stages. The activity of proline and peroxidase of eight varieties (Missouri, Yaqui, Vita, Feroz, Rio Grande, Tropic, Ace, and Floradade) submitted to NaCl concentrations (0, 50, 100, 150 and 200 mM de NaCl) and the semi-quantitative activity of 19 enzymes APY ZYM® were measured under a completely randomized design with four replications. Data were analyzed using univariate-multivariate análisis of variance, Tukey’s HSD (p = 0.05), canonical discriminant and cluster analysis. The results showed significant di_erences between varieties and NaCl in proline content. Proline increased as the NaCl concentration increased. Peroxidase did no show significant di_erences. Eight enzymes were included within the model to properly classify the varieties and NaCl. In shoots, varieties and NaCl showed that enzymatic activity was higher in the order of alkaline-phosphatase > leucine arylamidase > acid phosphatase > naphthol-AS-BI-phosphohydrolase > n-acetyl-_-glucosaminidase > _-galactosidase, while in roots was higher in the order of alkaline-phosphatase > naphthol-AS-BI-phosphohydrolase > acid phosphatase > n-acetyl-_-glucosaminidase. Acid and alkali phosphatase, lipase, esterase, _-galactosidase, and trypsin can be a potential biomarker for NaCl-stress tolerance in tomato." | |
Multidisciplinary Digital Publishing Institute | |
2019 | |
Artículo | |
Molecules | |
Inglés | |
FISIOLOGÍA VEGETAL | |
Versión publicada | |
publishedVersion - Versión publicada | |
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