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http://cibnor.repositorioinstitucional.mx/jspui/handle/1001/1834| Transcriptomic analysis of Pacific white shrimp (Litopenaeus vannamei, Boone 1931) in response to acute hepatopancreatic necrosis disease caused by Vibrio parahaemolyticus | |
| Adrián Esteban Velázquez Lizárraga JOSE LUIS JUAREZ MORALES Ilie Sava Racotta Dimitrov Humberto Villarreal Colmenares OSWALDO VALDES LOPEZ ANTONIO LUNA GONZALEZ María del Carmen Rodríguez Jaramillo NORMA ANGELICA ESTRADA MUÑOZ Felipe de Jesús Ascencio Valle | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| DOI: 10.1371/journal. pone.0220993 ISSN: 19326203 URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0220993 | |
| Vibrio parahaemolyticus, hepatopancreatic necrosis disease | |
| "Acute hepatopancreatic necrosis disease (AHPND), caused by marine bacteria Vibrio Parahaemolyticus, is a huge problem in shrimp farms. The V. parahaemolyticus infecting material is contained in a plasmid which encodes for the lethal toxins PirABVp, whose primary target tissue is the hepatopancreas, causing sloughing of epithelial cells, necrosis, and massive hemocyte infiltration. To get a better understanding of the hepatopancreas response during AHPND, juvenile shrimp Litopenaeus vannamei were infected by immersion with V. parahaemolyticus. We performed transcriptomic mRNA sequencing of infected shrimp hepatopancreas, at 24 hours post-infection, to identify novel differentially expressed genes a total of 174,098 transcripts were examined of which 915 transcripts were found differentially expressed after comparative transcriptomic analysis: 442 up-regulated and 473 downregulated transcripts. Gene Ontology term enrichment analysis for up-regulated transcripts includes metabolic process, regulation of programmed cell death, carbohydrate metabolic process, and biological adhesion, whereas for down-regulated transcripts include, microtubule- based process, cell activation, and chitin metabolic process. The analysis of proteinprotein network between up and down-regulated genes indicates that the first gene interactions are connected to oxidation-processes and sarcomere organization. Additionally, protein-protein networks analysis identified 20-top highly connected hub nodes. Based on their immunological or metabolic function, ten candidate transcripts were selected to measure their mRNA relative expression levels in AHPND infected shrimp hepatopancreas by RTqPCR. Our results indicate a close connection between the immune and metabolism systems during AHPND infection. Our RNA-Seq and RT-qPCR data provide the posible immunological and physiological scenario as well as the molecular pathways that take place in the shrimp hepatopancreas in response to an infectious disease." | |
| Public Library of Science | |
| 2019 | |
| Artículo | |
| PLoS ONE | |
| Inglés | |
| Velázquez-Lizárraga AE, Juárez-Morales JL, Racotta IS, Villarreal-Colmenares H, Valdes- Lopez O, Luna-González A, et al. (2019) Transcriptomic analysis of Pacific white shrimp (Litopenaeus vannamei, Boone 1931) in response to acute hepatopancreatic necrosis disease caused by Vibrio parahaemolyticus. PLoS ONE 14(8): e0220993. https://doi.org/10.1371/journal. pone.0220993 | |
| BACTERIOLOGÍA | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Artículos |
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