Por favor, use este identificador para citar o enlazar este ítem:
http://cibnor.repositorioinstitucional.mx/jspui/handle/1001/1833| Biochemical andmolecular characterization of N66 from the shell of Pinctada mazatlanica | |
| Crisalejandra Rivera Pérez CATALINA MAGALLANES DOMINGUEZ Josafat Jehú Ojeda Ramírez de Areyano NORMA YOLANDA HERNANDEZ SAAVEDRA | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| DOI: 10.7717/peerj.7212 ISSN: 21678359 URL: https://peerj.com/articles/7212/ | |
| "Mollusk shell mineralization is a tightly controlled process made by shell matrix proteins (SMPs). However, the study of SMPs has been limited to a few model species. In this study, the N66 mRNA of the pearl oyster Pinctada mazatlanica was cloned and functionally characterized. The full sequence of the N66 mRNA comprises 1,766 base pairs, and encodes one N66 protein. A sequence análisis revealed that N66 contained two carbonic anhydrase (CA) domains, a NG domain and several glycosylation sites. The sequence showed similarity to the CA VII but also with its homolog protein nacrein. The native N66 protein was isolated from the Shell and identified by mass spectrometry, the peptide sequence matched to the nucleotide sequence obtained. Native N66 is a glycoprotein with a molecular mass of 60–66 kDa which displays CA activity and calcium carbonate precipitation ability in presence of different salts. Also, a recombinant form of N66 was produced in Escherichia coli, and functionally characterized. The recombinant N66 displayed higher CA activity and crystallization capability than the native N66, suggesting that the lack of posttranslational modifications in the recombinant N66 might modulate its activity. " | |
| PeerJ | |
| 2019 | |
| Artículo | |
| PeerJ | |
| Inglés | |
| Rivera-Perez C, Magallanes-Dominguez C, Dominguez-Beltran RV, Ojeda-Ramirez de Areyano JJ, Hernandez- Saavedra NY. 2019. Biochemical and molecular characterization of N66 from the shell of Pinctada mazatlanica. PeerJ 7:e7212 DOI 10.7717/peerj.7212 | |
| GENÉTICA ANIMAL | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Artículos |
Cargar archivos:
| Fichero | Tamaño | Formato | |
|---|---|---|---|
| PUB-ARTICULO-4430.PDF | 899.5 kB | Adobe PDF | Visualizar/Abrir |