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http://cibnor.repositorioinstitucional.mx/jspui/handle/1001/1565| Molecular characterization and phylogenetic analysis of a Squash leaf curl virus isolate from Baja California Sur, Mexico | |
| Diana Medina Hernández María Goretty Caamal Chan MAYELA VARGAS SALINAS ABRAHAM LOERA MURO Aarón Barraza Celis RAMON JAIME HOLGUIN PEÑA | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| DOI: 10.7717/peerj.6774 ISSN: 2167-8359 URL: https://peerj.com/articles/6774/ | |
| Leaf curl disease, Iterons, Recombination, Genetic stability, Squash clade | |
| "Background. The begomovirus, squash leaf curl virus (SLCuV) is one of the causal agents of squash leaf curl (SLC) disease, which is among the most destructive diseases of cucurbit crops in tropical, subtropical, and semiarid regions worldwide. This disease was originally reported in the American continent with subsequent spread to the Mediterranean basin. Up to now, SLCuV has only been detected by PCR in Mexico. This study provides the first complete sequence of a Mexican SLCuV isolate from Baja California Sur (BCS). In addition, the genome of the virus was characterized, establishing its phylogenetic relationship with other SLCuV isolates. Methods. The full genome (DNA-A and DNA-B) was amplified by rolling circle amplification, cloned and sequenced and the open reading frames (ORF) were annotated.Virus identification was performed according to the International Committee on Taxonomy of Viruses (ICTV) criteria for begomovirus species demarcation. To infer evolutionary relationship with other SLCuV isolates, phylogenetic and recombination analyses were performed. Results. The SLCuV-[MX-BCS-La Paz-16] genome (DNA-A and DNA-B) had 99% identity with SLCuV reference genomes. The phylogenetic analysis showed that SLCuV- [MX-BCS-La Paz-16] is closely related to SLCuV isolates from the Middle East (Egypt, Israel, Palestine and Lebanon). No evidence of interspecific recombination was determined and iterons were 100% identical in all isolates in the SLCuV clade. Conclusions. SLCuV-[MX-BCS-La Paz-16] showed low genetic variability in its genome, which could be due to a local adaptation process (isolate environment), suggesting that SLCuV isolates from the Middle East could have derived from the southwestern United States of America (USA) and northwestern Mexico." | |
| 2019 | |
| Artículo | |
| Peer J | |
| Inglés | |
| Medina-Hernández D, Caamal-Chan MG, Vargas-Salinas M, Loera-Muro A, Barraza A, Holguín-Peña RJ. 2019. Molecular characterization and phylogenetic analysis of a Squash leaf curl virus isolate from Baja California Sur, Mexico. PeerJ 7:e6774https://doi.org/10.7717/peerj.6774 | |
| VIRUS | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Artículos |
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| medina_d PeerJ2019.pdf | 3.6 MB | Adobe PDF | Visualizar/Abrir |