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http://cibnor.repositorioinstitucional.mx/jspui/handle/1001/931| In vitro Digestibility of Yarrowia lipolytica Yeast and Growth Performance in Whiteleg Shrimp Litopenaeus vannamei | |
| ANA RUTH ALVAREZ SANCHEZ CLAUDIO HUMBERTO MEJIA RUIZ Héctor Gerardo Nolasco Soria Alberto Peña Rodríguez | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| DOI: 10.4194/1303-2712-v18_3_05 URL: http://www.trjfas.org ISSN: 1303-2712 | |
| Marine yeast, cell digestibility, cell viability, turbidimetry, reduced sugars | |
| "Marine yeasts used in aquaculture disease control can also be an important protein source for improving feeding and nutrition of crustaceans. Yarrowia lipolyticca has been studied for its capacity to secrete heterologous proteins and high content of unsaturated fatty acids, beta-glucan, and mannane polymers in the cell wall. We measured in vitro digestibility of Y. lipolyticca by whiteleg shrimp Litopenaeus vannamei digestive enzymes, and an in vivo assay of Y. lipolytica in feed onwhiteleg shrimp growth. We found that digestive gland enzymes of shrimp digest Y. lipolytica, based on reduced optical density of a yeast suspension. Digestion was –0.00236 ± 0.00010 OD U min–1 for intact cells and –0.00325 ± 0.00010 OD U min–1 for lysed cells. Release of reducing sugars in intact cells (5.3940 ± 0.1713 μmol h–1), and lysed cells (0.8396 ± 0.2251 μmol h–1) was measured. Digestive gland treatment significantly reduced cell viability (near 100%), relative to the control. Electron microscopy shows that the cell wall of Y. lipolytica exposed to the digestive gland enzymes was severely damaged. Shrimp diet containing Y. lipolytica resulted in significantly higher weight gain and specific growth rate of whiteleg shrimp." | |
| Central Fisheries Research Institute, Trabzon, Turkey | |
| 2018 | |
| Artículo | |
| Turkish Journal of Fisheries and Aquatic Sciences | |
| Inglés | |
| BIOQUÍMICA DE ALIMENTOS | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Artículos |
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| Fichero | Tamaño | Formato | |
|---|---|---|---|
| Turkish Journal of Fisheries and Aquatic Sciences 2018 v18 pp395-404 LINK.PDF | 11.34 kB | Adobe PDF | Visualizar/Abrir |