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Isolation and Characterization of 26 Microsatellite Loci for the Shortfin Silverside Fish Chirostoma humboldtianum Valenciennes 1835 (Atherinopsidae: Menidiinae) Derived from Next Generation Sequencing and Their Cross-Amplification in Central Mexican Atherinopsids
IRENE DE LOS ANGELES BARRIGA SOSA
ROSA MARIA GARCIA MARTINEZ
JHOANA DIAZ LARREA
OSCAR ADRIAN LOZANO GARZA
FRANCISCO JAVIER GARCIA DE LEON
Acceso Abierto
Atribución-NoComercial-SinDerivadas
DOI: 10.4236/ojgen.2014.45032
ISSN: 2162-4461
URL: http://www.scirp.org/journal/ojgen
Endemic, Polymorphism, Villa Victoria, Poblana alchichica, C. promelas, C. riojai
"The endemic silverside fish C. humboldtianum is of great ichtyologycal, economical and cultural relevance in central Mexico and it has been suggested that it is among a group of other “peces blancos”, the most ancestral species. Here we characterized a set of 26 microsatellite loci from the species in order to further assess population and phylogeographic issues that aid in evaluating their highly impacted populations. From 58 primer pairs tested on specimens from Villa Victoria dam (Rio Balsas Basin), 26 loci positively amplified on the species and cross-primed on specimens of the closely related and threatened Poblana alchichica, C. promelas and C. riojai. Loci resolved medium and high genetic variation (mean NA = 14.3, SD = 9.38; mean HO & HE = 0.47, SD = 0.32 and 0.58, SD = 0.32, respectively) and it is expected that these polymorphic loci are also useful in studing threatened atherinopsid species. "
Scientific Research Publishing, Inc.
2014
Artículo
Open Journal of Genetics
Inglés
Angeles, B. , García-Martínez, R. , Díaz-Larrea, J. , Garza, O. and García-De-León, F. (2014) Isolation and Characterization of 26 Microsatellite Loci for the Shortfin Silverside Fish Chirostoma humboldtianum Valenciennes1835 (Atherinopsidae: Menidiinae) Derived from Next Generation Sequencing and Their Cross-Amplification in Central Mexican Atherinopsids. Open Journal of Genetics, 4, 355-361.
GENÉTICA ANIMAL
Versión publicada
publishedVersion - Versión publicada
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